Department of Chemical Engineering, University of Saskatchewan, Saskatoon, Saskatchewan Canada.
Biotechnol Bioeng. 1992 Jun 5;40(1):63-8. doi: 10.1002/bit.260400110.
Recovery of rapeseed proteins from defatted canola meal by precipitation was investigated. The ability of different precipitating agents, such as sodium hexametaphosphate (HMP), carboxymethylcellulose (CMC), ammonium sulphate, and isoelectric precipitation using HCl, were evaluated based on the yield and mean size of protein aggregates. Almost 94% of dissolved protein was precipitated in the presence of 2.7 M ammonium sulphate, while the largest mean protein particle size (32 microm) was obtained in the presence of HMP at pH 3.3.
通过沉淀从脱油菜籽粕中回收菜籽蛋白。考察了不同沉淀剂（六偏磷酸钠（HMP）、羧甲基纤维素（CMC）、硫酸铵和盐酸等电沉淀）的沉淀能力，根据蛋白质聚集体的产率和平均粒径进行评价。在 2.7 M 硫酸铵存在下，几乎 94%的溶解蛋白被沉淀，而在 pH 3.3 时 HMP 存在下获得的蛋白质颗粒平均粒径最大（32 微米）。
