Watanabe Wataru, Matsunaga Sachihiro, Higashi Tsunehito, Fukui Kiichi, Itoh Kazuyoshi
National Institute of Advanced Industrial Science and Technology, Photonics Research Institute, 1-8-31, Midorigaoka, Ikeda, Osaka 563-8577, Japan.
J Biomed Opt. 2008 May-Jun;13(3):031213. doi: 10.1117/1.2939401.
Femtosecond laser pulses in the near-infrared region have potential applications in the imaging and manipulation of intracellular organelles. We report on the manipulation of intracellular organelles by two-photon excitation. The dynamics of green fluorescent protein (GFP)-histone are investigated by two-photon fluorescence recovery after photobleaching (FRAP). Intracellular ablation of fluorescently labeled organelles in living cells is performed by focusing femtosecond laser pulses. We report on the selective marking of individual organelles by using two-photon conversion of a photoconvertible fluorescent protein.
近红外区域的飞秒激光脉冲在细胞内细胞器的成像和操控方面具有潜在应用。我们报告了通过双光子激发对细胞内细胞器的操控。通过光漂白后双光子荧光恢复（FRAP）研究了绿色荧光蛋白（GFP）-组蛋白的动力学。通过聚焦飞秒激光脉冲对活细胞中荧光标记的细胞器进行细胞内消融。我们报告了利用光转换荧光蛋白的双光子转换对单个细胞器进行选择性标记的情况。
