Sfrent-Cornateanu Roxana, Mihai Carina, Stoian Irina, Lixandru Daniela, Bara Constantin, Moldoveanu Elena
Department of Physiopathology and Immunology, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Clin Chem Lab Med. 2008;46(6):836-41. doi: 10.1515/CCLM.2008.132.
Oxidative stress is associated with scleroderma (systemic sclerosis) and is supposed to favor disease progression by complex effects on the vascular endothelium and on fibroblasts.
Plasma oxidative process marker, thiobarbituric acid-reactive substances, and several markers of antioxidant defense capacity (plasma total antioxidant activity, serum albumin, uric acid and glutathione, superoxide dismutase and catalase) were evaluated by spectrophotometric methods using blood samples collected from 23 scleroderma patients and 21 healthy controls.
In scleroderma patients, thiobarbituric acid-reactive substances levels (mmol/L plasma) were significantly elevated (29.3+/-5.8) compared with healthy controls (16.6+/-3.1, p<0.001). Total antioxidant activity (mmol Trolox/L) was significantly lower in scleroderma patients than in controls (1.29+/-0.13 vs. 1.55+/-0.23, p<0.001), as well as the antioxidant gap (mmol Trolox/L) (0.57+/-0.18 vs. 0.92+/-0.22, p<0.001). Superoxide dismutase activity (IU/g hemoglobin) was markedly decreased in patients as compared with controls (395+/-184 vs. 659+/-211, p<0.001).
Lower plasma total antioxidant activity and plasma antioxidant gap in scleroderma patients show that plasma antioxidant defense is deficient in scleroderma patients. As previous studies on this issue are controversial, the decreased erythrocyte superoxide dismutase activity found in the patients in this study needs further investigation.
氧化应激与硬皮病（系统性硬化症）相关，并且被认为通过对血管内皮和成纤维细胞的复杂作用促进疾病进展。
采用分光光度法，对从23例硬皮病患者和21例健康对照者采集的血样进行检测，评估血浆氧化过程标志物硫代巴比妥酸反应性物质，以及几种抗氧化防御能力标志物（血浆总抗氧化活性、血清白蛋白、尿酸和谷胱甘肽、超氧化物歧化酶和过氧化氢酶）。
与健康对照者相比，硬皮病患者的硫代巴比妥酸反应性物质水平（血浆mmol/L）显著升高（29.3±5.8），而健康对照者为（16.6±3.1，p<0.001）。硬皮病患者的总抗氧化活性（mmol Trolox/L）显著低于对照者（1.29±0.13对1.55±0.23，p<0.001），抗氧化差值（mmol Trolox/L）也是如此（0.57±0.18对0.92±0.22，p<0.001）。与对照者相比，患者的超氧化物歧化酶活性（IU/g血红蛋白）显著降低（395±184对659±211，p<0.001）。
硬皮病患者血浆总抗氧化活性和血浆抗氧化差值降低，表明硬皮病患者血浆抗氧化防御功能不足。由于此前关于该问题的研究存在争议，本研究中患者红细胞超氧化物歧化酶活性降低这一发现需要进一步研究。
