使用PCR-RFLP分析法对西澳大利亚州猪体内等孢球虫属的分子鉴定及流行情况
Molecular identification and prevalence of Isospora sp. in pigs in Western Australia using a PCR-RFLP assay.
作者信息
Johnson Johanna, Samarasinghe Bimba, Buddle Ross, Armson Anthony, Ryan Una
机构信息
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
出版信息
Exp Parasitol. 2008 Oct;120(2):191-3. doi: 10.1016/j.exppara.2008.06.005. Epub 2008 Jun 14.
Little is known about the prevalence of Isospora in domestic pigs in Western Australia. A total of 289 pig faecal samples were collected from pre- and post-weaned pigs and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of Western Australia. Faecal samples were screened using a PCR-RFLP assay based on the ITS-1 rDNA locus. An overall prevalence of 10.4% (30/289) was identified. Isospora was detected in 16.3% (20/123) of pre-weaned animals and 6.4% (10/156) of post-weaned animals. PCR-RFLP analysis confirmed the presence of Isospora suis in 86.7% of the positive Isospora isolates. Isospora was significantly associated with diarrhea and the findings of this study suggest that management factors such as cleaning practices, flooring types and stocking densities need to be investigated in the porcine host to find new and improved measures for control.
西澳大利亚州家猪中艾美耳球虫的流行情况鲜为人知。从西澳大利亚州西南部地区的1个室内和3个室外养猪场的断奶前和断奶后仔猪及母猪中总共采集了289份猪粪便样本。粪便样本采用基于ITS-1 rDNA基因座的PCR-RFLP分析方法进行筛查。总体流行率为10.4%（30/289）。在16.3%（20/123）的断奶前动物和6.4%（10/156）的断奶后动物中检测到艾美耳球虫。PCR-RFLP分析证实86.7%的阳性艾美耳球虫分离株中存在猪艾美耳球虫。艾美耳球虫与腹泻显著相关，本研究结果表明，需要在猪宿主中调查清洁措施、地板类型和饲养密度等管理因素，以找到新的和改进的控制措施。
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