Johnson Johanna, Buddle Ross, Reid Simon, Armson Anthony, Ryan Una M
Division of Health Sciences, School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
Exp Parasitol. 2008 Jul;119(3):418-21. doi: 10.1016/j.exppara.2008.04.009. Epub 2008 Apr 14.
A total of 289 pig faecal samples were collected from pre-weaned and post-weaned piglets and sows from 1 indoor and 3 outdoor piggeries located in the south-west region of Western Australia and screened at the 18S rRNA locus for the presence of Cryptosporidium. An overall prevalence of 22.1% (64/289) was identified. Cryptosporidium was more prevalent in post-weaned animals (p<0.05); 32.7% (51/156) versus 10.6% (13/123) for pre-weaned animals. Sequence analysis identified Cryptosporidium suis in all pre-weaned isolates genotyped (7/13). In post-weaned pigs that were genotyped (n=38), the non-zoonotic Cryptosporidium species, pig genotype II was identified in 32 samples and C. suis in 6 samples. The zoonotic species Cryptosporidium parvum was not detected, suggesting that domestic pigs do not pose a significant public health risk. Pig genotype II was significantly (p<0.05) associated with 'normal' stools, indicating an asymptomatic nature in the porcine host.
从西澳大利亚州西南部的1个室内和3个室外养猪场的断奶前和断奶后仔猪以及母猪中总共采集了289份猪粪便样本，并在18S rRNA位点筛查隐孢子虫的存在。总体患病率为22.1%（64/289）。隐孢子虫在断奶后动物中更为普遍（p<0.05）；断奶前动物为10.6%（13/123），断奶后动物为32.7%（51/156）。序列分析在所有进行基因分型的断奶前分离株（7/13）中鉴定出猪隐孢子虫。在进行基因分型的断奶后猪（n = 38）中，在32个样本中鉴定出非人畜共患的隐孢子虫物种猪基因型II，在6个样本中鉴定出猪隐孢子虫。未检测到人畜共患物种微小隐孢子虫，这表明家猪不会构成重大的公共卫生风险。猪基因型II与“正常”粪便显著相关（p<0.05），表明在猪宿主中具有无症状的性质。
