Kim Su-Mi, Lee Kwang-Nyeong, Park Jee-Yong, Ko Young-Joon, Joo Yi-Seok, Kim Hyun-Soo, Park Jong-Hyeon
Foreign Animal Disease Division, National Veterinary Research and Quarantine Service, Manan-Gu, Anyang, Gyeonggi-Do, Republic of Korea.
Antiviral Res. 2008 Nov;80(2):178-84. doi: 10.1016/j.antiviral.2008.06.001. Epub 2008 Jul 2.
Foot-and-mouth disease (FMD) is an economically important animal disease because of the speed of its transmission. Routine vaccination may not be effective for early protection in an outbreak situation. Small interfering RNA (siRNA) can be used in a rapid and effective antiviral approach. However, siRNA has limitations when used in disease prevention, such as a short duration of action. In this study, we have demonstrated that treatment with siRNA after FMD virus (FMDV) infection has an antiviral effect and could be effective in control of FMDV. We applied adenoviruses expressing siRNA both before and after FMDV infection in vitro and in vivo. Treatment after FMDV infection gave effective viral inhibition, but a combination of treatment before and after FMDV infection gave the best results in IBRS-2 cells. We obtained high survival rates in suckling mice by the use of therapeutic injections following challenge. The results of this study suggest that treatment with siRNA could enhance antiviral effects and may be helpful in the control of FMDV in an outbreak.
口蹄疫（FMD）因其传播速度快而成为一种具有重要经济影响的动物疾病。在疫情爆发情况下，常规疫苗接种可能无法提供早期有效保护。小干扰RNA（siRNA）可用于一种快速有效的抗病毒方法。然而，siRNA在疾病预防应用中存在局限性，比如作用持续时间短。在本研究中，我们证明了在口蹄疫病毒（FMDV）感染后用siRNA进行治疗具有抗病毒作用，并且对控制FMDV可能有效。我们在体外和体内FMDV感染之前和之后都应用了表达siRNA的腺病毒。FMDV感染后进行治疗可有效抑制病毒，但在IBRS - 2细胞中，FMDV感染前后联合治疗效果最佳。通过在攻毒后进行治疗性注射，我们在乳鼠中获得了高存活率。本研究结果表明，用siRNA进行治疗可增强抗病毒效果，可能有助于在疫情爆发时控制FMDV。
