恒河猴5号染色体的高分辨率辐射杂种图谱鉴定出基因组组装中的重排。
A high-resolution radiation hybrid map of rhesus macaque chromosome 5 identifies rearrangements in the genome assembly.
作者信息
Karere Genesio M, Froenicke Lutz, Millon Lee, Womack James E, Lyons Leslie A
机构信息
Department of Population Health and Reproduction, School of Veterinary Medicine, California National Primate Research Center, Davis, CA 95616, USA.
出版信息
Genomics. 2008 Oct;92(4):210-8. doi: 10.1016/j.ygeno.2008.05.013. Epub 2008 Jul 31.
Abstract
A 10,000-rad radiation hybrid (RH) cell panel of the rhesus macaque was generated to construct a comprehensive RH map of chromosome 5. The map represents 218 markers typed in 185 RH clones. The 4846-cR map has an average marker spacing of 798 kb. Alignments of the RH map to macaque and human genome sequences confirm a large inversion and reveal a previously unreported telomeric inversion. The macaque genome sequence indicates small translocations from the ancestral homolog of macaque chromosome 5 to macaque chromosomes 1 and 6. The RH map suggests that these are probably assembly artifacts. Unlike the genome sequence, the RH mapping data indicate the conservation of synteny between macaque chromosome 5 and human chromosome 4. This study shows that the 10,000-rad panel is appropriate for the generation of a high-resolution whole-genome RH map suitable for the verification of the rhesus genome assembly.
摘要
构建了一个恒河猴的10000拉德辐射杂种（RH）细胞系，用于构建5号染色体的综合RH图谱。该图谱代表了在185个RH克隆中分型的218个标记。4846厘镭图谱的平均标记间距为798 kb。RH图谱与猕猴和人类基因组序列的比对证实了一个大的倒位，并揭示了一个以前未报道的端粒倒位。猕猴基因组序列表明，从猕猴5号染色体的祖先同源物到猕猴1号和6号染色体存在小的易位。RH图谱表明这些可能是组装假象。与基因组序列不同，RH定位数据表明猕猴5号染色体和人类4号染色体之间存在同线性保守。这项研究表明，10000拉德细胞系适合用于生成适用于验证恒河猴基因组组装的高分辨率全基因组RH图谱。
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