Truong Dinh Quang, Cornet Anne, Wieërs Grégoire, Robert Annie, Reding Raymond, Latinne Dominique
Pediatric Liver Transplant Program, Saint-Luc University Clinics, Université catholique de Louvain, Brussels, Belgium.
Transpl Immunol. 2008 Jul;19(3-4):215-9. doi: 10.1016/j.trim.2008.06.001. Epub 2008 Jul 2.
This study aims to investigate potential role of granzyme B enzyme-linked immunosorbent spot (GrB ELISPOT) for immunological monitoring in pediatric liver transplantation.
Peripheral blood mononuclear cells from 28 pediatric recipients were serially tested for GrB-producing donor-reactive cells at day 0 pre-transplantation (baseline) and days 7, 14, and 28 post-transplantation.
At baseline, no difference of GrB value was found in acute rejection (14/28) compared to normal graft function patients (day 0: 4(3.9) spots versus 5(2.9) spots, respectively: p=0.65). At day 7 post-transplantation, acute rejection patients showed frequencies of GrB ELISPOT higher than those with normal graft function, but the differences observed were not statistically significant (day 7: 15(4.9) spots versus 10(4.0) spots, respectively: p=0.55). GrB increased significantly at day 7 from baseline in the rejection group (15(4.9) spots versus 4(3.9), respectively p=0.04), whereas corresponding changes were not significant in the group without rejection (10(4.0) versus 5(2.9), respectively: p=0.15).
GrB ELISPOT pre-transplantation could not predict the occurrence of early post-transplant acute rejection; similarly frequencies at days 7, 14 and 28 could not be correlated with acute rejection in pediatric liver recipients. However, a kinetic study of GrB ELISPOT could be helpful to predict or confirm early rejection in the small group of liver allograft recipients analyzed in this study.
本研究旨在探讨颗粒酶B酶联免疫斑点法（GrB ELISPOT）在小儿肝移植免疫监测中的潜在作用。
对28例小儿肝移植受者的外周血单个核细胞在移植前第0天（基线）以及移植后第7、14和28天进行连续检测，以检测产生GrB的供体反应性细胞。
在基线时，急性排斥反应患者（14/28）与移植肝功能正常患者相比，GrB值无差异（第0天：分别为4（3.9）个斑点和5（2.9）个斑点，p = 0.65）。移植后第7天，急性排斥反应患者的GrB ELISPOT频率高于移植肝功能正常者，但观察到的差异无统计学意义（第7天：分别为15（4.9）个斑点和10（4.0）个斑点，p = 0.55）。排斥反应组中，GrB在第7天较基线时显著增加（分别为15（4.9）个斑点和4（3.9）个斑点，p = 0.04），而无排斥反应组的相应变化不显著（分别为10（4.0）个斑点和5（2.9）个斑点，p = 0.15）。
移植前GrB ELISPOT无法预测移植后早期急性排斥反应的发生；同样，第7、14和28天的频率与小儿肝移植受者的急性排斥反应也无相关性。然而，GrB ELISPOT的动力学研究可能有助于预测或确认本研究中分析的一小部分肝移植受者的早期排斥反应。
