肥胖高血糖小鼠脂肪组织中的三酰甘油生物合成
Triacylglycerol biosynthesis in the adipose tissue of the obese-hyperglycaemic mouse.
作者信息
Jamdar S C, Shapiro D, Fallon H J
出版信息
Biochem J. 1976 Aug 15;158(2):327-34. doi: 10.1042/bj1580327.
Abstract
Obesity in obese-hyperglycaemic mouse is associated with an increase in number and size of adipocytes. Adipocytes from the obese mouse showed increased incorporation of [14C]acetate and[14C]glucose into triacylglycerol. This increased capacity of triacylglycerol formation was correlated with increased activities of various triacylglycerol-forming enzymes measured in the microsomal fraction of adipose tissue from obese mice. Microsomal fractions from lean and obese mice contained sn-glycerol 3-phosphate acyltransferase, phosphatidate phosphohydrolase and diacylglycerol acyltransferase. Phosphatidate phosphohydrolase was also detected in the soluble fraction. In the presence of Mg2+, the phosphatidate phsophohydrolase from the soluble and the microsomal fractions was active towards membrane-bound phosphatidate. Among the three enzymes studied here, the increase in Mg2+-dependent phosphatidate phosphohydrolase was most prominent in adipose tissue of obese mice.
摘要
肥胖高血糖小鼠的肥胖与脂肪细胞数量增加及体积增大有关。肥胖小鼠的脂肪细胞显示出[14C]乙酸盐和[14C]葡萄糖掺入三酰甘油的量增加。这种三酰甘油形成能力的增强与在肥胖小鼠脂肪组织微粒体部分测得的各种三酰甘油形成酶活性增加相关。瘦小鼠和肥胖小鼠的微粒体部分含有sn-甘油3-磷酸酰基转移酶、磷脂酸磷酸水解酶和二酰甘油酰基转移酶。在可溶部分也检测到了磷脂酸磷酸水解酶。在Mg2+存在的情况下，来自可溶部分和微粒体部分的磷脂酸磷酸水解酶对膜结合的磷脂酸有活性。在这里研究的三种酶中，Mg2+依赖的磷脂酸磷酸水解酶在肥胖小鼠的脂肪组织中增加最为显著。
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