Suzuki Yuji, Mae Tadahiko, Makino Amane
Laboratory of Plant Environmental Responses, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Biosci Biotechnol Biochem. 2008 Jul;72(7):1951-3. doi: 10.1271/bbb.80084. Epub 2008 Jul 7.
High-quality total RNA was extracted using a cethyltrimethylammonium bromide-containing buffer followed by an acid guanidium thiocyanate-phenol-chloroform treatment from recalcitrant plant tissues such as tree leaves (pine, Norway spruce, ginkgo, Japanese cedar, rose), flowers (rose, Lotus japonicus) and storage tissues (seeds of Lotus japonicus and rice, sweet potato tuber, banana fruit). This protocol greatly reduced the time required for RNA extraction.
使用含十六烷基三甲基溴化铵的缓冲液，随后进行酸性硫氰酸胍-苯酚-氯仿处理，从难提取的植物组织中提取高质量的总RNA，这些组织包括树叶（松树、挪威云杉、银杏、日本柳杉、玫瑰）、花朵（玫瑰、百脉根）和贮藏组织（百脉根和水稻种子、甘薯块茎、香蕉果实）。该方案大大减少了RNA提取所需的时间。
