Aguzzi Jacopo, Sarriá David, García José A, Del Rio Joaquin, Sardà Francesc, Manuel Antoni
Institut de Ciències del Mar ICM- Passeig Marítim de la Barceloneta 37-49, 08003 Barcelona, Spain.
J Neurosci Methods. 2008 Aug 30;173(2):215-24. doi: 10.1016/j.jneumeth.2008.06.009. Epub 2008 Jun 20.
The Norway lobster, Nephrops norvegicus (L.), is a deep-water burrowing decapod of high commercial value. Diel variations in trawl captures are produced by population rhythms of burrow emergences related to day-night cycles. Rhythms seem to be different in males and females since catches show variations in sex ratios depending on the season. Our hypothesis is that the diel rhythm of activity in this species can be distinguished in three different behavioural sets, the durations of which show gender-related modulation: door-keeping, proximal-, and distal-emergence from the burrow. Our aim is to detail the functioning of a new tracking system allowing the durations of these three behavioural components to be determined. Movement of animals was detected by subdividing aquaria into different zones by means of three rows of infrared-emitting and -receiving photodiodes in which blue light emitters were also integrated for the generation of light cycles. We recorded movement patterns in adult males and females (n=20) exposed to a standard photoperiod regime (i.e., 12 h; monochromatic at 480 nm of 5 lx) over 12 days. Marked diel nocturnal rhythms were reported at all barriers, with activity peaks diffused over the night at the burrow entrance and located at the day-night transition at other barriers (i.e., crepuscular peaks that decreased in the next few hours of darkness). Mean total activity was significantly higher for females than males at the burrow entrance (i.e., door-keeping behaviour). Males had significantly higher activity at other locations (proximal- and distal-emergence behaviours).
挪威龙虾，学名Nephrops norvegicus (L.)，是一种具有较高商业价值的深水穴居十足目动物。拖网捕捞量的昼夜变化是由与昼夜周期相关的洞穴出现的种群节律产生的。由于渔获量显示出性别比例随季节变化，因此雄性和雌性的节律似乎有所不同。我们的假设是，该物种的活动昼夜节律可以分为三种不同的行为模式，其持续时间呈现出与性别相关的调节：守洞、洞穴近端出现和洞穴远端出现。我们的目的是详细描述一种新的跟踪系统的功能，该系统可以确定这三种行为成分的持续时间。通过三排红外发射和接收光电二极管将水族箱划分为不同区域来检测动物的运动，其中还集成了蓝光发射器以产生光周期。我们记录了成年雄性和雌性（n = 20）在12天内暴露于标准光周期条件下（即12小时；480 nm单色光，光照强度为5勒克斯）的运动模式。在所有障碍物处都观察到明显的夜间昼夜节律，洞穴入口处的活动高峰在夜间扩散，而在其他障碍物处则位于昼夜交替时（即黄昏高峰在随后的黑暗时段逐渐减弱）。在洞穴入口处（即守洞行为），雌性的平均总活动量显著高于雄性。在其他位置（近端和远端出现行为），雄性的活动量显著更高。
