Saito Y, Nakada Y, Hotta T, Mikami T, Kurisu K, Yamada K, Kiya K, Kawamoto K, Uozumi T
Department of Neurosurgery, Hiroshima University School of Medicine, Japan.
Int J Cancer. 1991 Jul 30;48(6):861-5. doi: 10.1002/ijc.2910480612.
Three ACNU-resistant sublines (R1, R3 and R12) from rat glioma 9L cells showed cross-resistance to vinblastine, adriamycin, and VP-16. Among these, the R3 subline also acquired radioresistance under aerobic conditions. Total glutathione levels in these sublines were elevated 2- to 3-fold. Treatment of the cells with BSO, a specific inhibitor of GSH synthesis, resulted in decreased intracellular total glutathione levels in all 4 cell lines to about 10% of control levels. However, sensitivity to radiation or to chemicals did not change accordingly. Treatment of 9L cells with OTZ, a precursor of cysteine, resulted in a rise in intracellular GSH levels but it did not correlate with sensitivity to X-ray or to ACNU. These results suggest that, in terms of cellular sensitivity to radiation or ACNU, total glutathione level alone cannot serve as a predictive indicator.
来自大鼠神经胶质瘤9L细胞的三个对ACNU耐药的亚系（R1、R3和R12）对长春花碱、阿霉素和VP - 16表现出交叉耐药性。其中，R3亚系在有氧条件下还获得了辐射抗性。这些亚系中的总谷胱甘肽水平提高了2至3倍。用BSO（一种谷胱甘肽合成的特异性抑制剂）处理细胞，导致所有4种细胞系中的细胞内总谷胱甘肽水平降至对照水平的约10%。然而，对辐射或化学物质的敏感性并未相应改变。用半胱氨酸前体OTZ处理9L细胞，导致细胞内谷胱甘肽水平升高，但这与对X射线或ACNU的敏感性无关。这些结果表明，就细胞对辐射或ACNU的敏感性而言，仅总谷胱甘肽水平不能作为预测指标。
