Harizanis P-C, Alissandrakis E, Tarantilis P-A, Polissiou M
Laboratory of Sericulture and Apiculture, Agricultural University of Athens, Athens, Greece.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Oct;25(10):1272-7. doi: 10.1080/02652030802007546.
Protection of honeycombs from the Wax moth, Galleria mellonella, involves the use of physical, biological or chemical control methods. As chemical control may result in residues in the extracted honey, the presence of p-dichlorobenzene and naphthalene residues was investigated by solid-phase microextraction (SPME) coupled to gas-chromatographic/mass spectrometry (GC/MS). The method was linear between 5 and 200 microg kg(-1) honey for p-dichlorobenzene and 1 and 200 microg kg(-1) for naphthalene. Limits of detection were 1 and 0.1 microg kg(-1), respectively, for p-dichlorobenzene and naphthalene, while relative standard deviations were 2.6 and 7.9%, respectively. Application of the method to 90 unifloral Greek honeys revealed that, in 25.6% of the samples, the concentration of either one of the pesticides exceeded the maximum residue level (MRL). Maximum concentrations were 163.03 microg kg(-1) honey for p-dichlorobenzene and 193.74 microg kg(-1) honey for naphthalene. Naphthalene was found in traceable amounts in 78.9% of the samples, but only 5.6% of them contained concentrations above the MRL, which indicates the use of pre-contaminated honeycomb foundations or built combs. Nevertheless, because naphthalene is naturally present in some plant species growing in Greece, the contribution of nectar from such a floral source should not be overlooked.
保护蜂巢免受蜡螟（大蜡螟）侵害涉及使用物理、生物或化学防治方法。由于化学防治可能导致提取的蜂蜜中残留，因此采用固相微萃取（SPME）结合气相色谱/质谱联用（GC/MS）技术对蜂蜜中对二氯苯和萘残留进行了研究。该方法对于对二氯苯在蜂蜜中的线性范围为5至200 μg kg⁻¹，对于萘为1至200 μg kg⁻¹。对二氯苯和萘的检测限分别为1和0.1 μg kg⁻¹，相对标准偏差分别为2.6%和7.9%。将该方法应用于90份希腊单花蜂蜜，结果显示，在25.6%的样品中，两种农药中任一种的浓度超过了最大残留限量（MRL）。对二氯苯在蜂蜜中的最大浓度为163.03 μg kg⁻¹，萘为193.74 μg kg⁻¹。在78.9%的样品中检测到痕量萘，但其中只有5.6%的样品萘浓度高于MRL，这表明使用了预先污染的蜂巢基础或已建成的蜂巢。然而，由于萘天然存在于希腊生长的一些植物物种中，来自此类花卉来源的花蜜的贡献不应被忽视。
