Buzzini Pietro, Turchetti Benedetta, Branda Eva, Goretti Marta, Amici Marco, Lagneau Paul Emile, Scaccabarozzi Licia, Bronzo Valerio, Moroni Paolo
Department of Applied Biology, University of Perugia, Italy.
Med Mycol. 2008 Aug;46(5):511-4. doi: 10.1080/13693780801993611.
A large scale screening of the in vitro susceptibility of 105 strains of Prototheca zopfii to a panel of polyene antibiotics (amphotericin B, nystatin, primaricin and filipin) was conducted. Strains studied were isolated from dairy-associated environments in five different localities. Groups 1-4 included strains recovered from four separate regions of Italy, while group 5 included isolates from Belgium. Amphotericin B and primaricin exhibited the highest activity, with th MIC90 ranging from 4 and 8 microg/ml, respectively. On the other hand, the MIC90 of nystatin and filipin were from two to four times higher. Two strains were resistant to all four polyenes tested. The above results are compared with those in the literature and the importance of carrying out large-scale screening surveys to assess polyene susceptibility patterns within the species P. zopfii is discussed.
对105株威克海姆原藻进行了一组多烯抗生素（两性霉素B、制霉菌素、匹马霉素和菲律宾菌素）的体外药敏大规模筛选。所研究的菌株从五个不同地区的乳制品相关环境中分离得到。第1 - 4组包括从意大利四个不同地区分离得到的菌株，而第5组包括来自比利时的分离株。两性霉素B和匹马霉素表现出最高活性，其MIC90分别为4和8微克/毫升。另一方面，制霉菌素和菲律宾菌素的MIC90高出两到四倍。有两株菌对所有四种测试的多烯类抗生素耐药。将上述结果与文献中的结果进行了比较，并讨论了开展大规模筛选调查以评估威克海姆原藻种内多烯药敏模式的重要性。
