Gruger Thomas, Morler Caroline, Schnitzler Norbert, Brandenburg Kerstin, Nidermajer Sabine, Horre Regine, Zundorf Josef
Federal Institute for Drugs and Medical Devices (BfArM), Biosafety Laboratory, Bonn, Germany.
Med Mycol. 2008 Nov;46(7):675-84. doi: 10.1080/13693780802017535.
Candida albicans infections often occur during or shortly after antibacterial treatment. Phagocytosis by polymorphonuclear neutrophil granulocytes (PMN) is the most important primarily defence mechanism against C. albicans. Certain antibiotics such as some fluoroquinolones (FQ) are known to influence phagocyte functions. Thus, we investigated the influence of older and newer FQ on the phagocytosis and killing of C. albicans by human PMN paying special attention to CD11b expression of these cells as an indicator of the degree of their activation. In order to obtain comprehensive and comparable results we tested 13 FQ over a wide range of concentrations and in a time dependent manner in a standardized approach. When used at therapeutic concentrations, the FQ tested did not influence to a clinically significant degree the phagocytosis or the killing of C. albicans by human PMN and also not their activation. However, at high concentrations those FQ with cyclopropyl-moiety at position N1 showed increase in CD11b expression and diminished phagocytosis and oxidative burst.
白色念珠菌感染常发生在抗菌治疗期间或治疗后不久。多形核中性粒细胞（PMN）的吞噬作用是抵御白色念珠菌的最重要的主要防御机制。某些抗生素，如一些氟喹诺酮类药物（FQ），已知会影响吞噬细胞功能。因此，我们研究了新旧两代FQ对人PMN吞噬和杀灭白色念珠菌的影响，并特别关注这些细胞的CD11b表达，将其作为细胞活化程度的指标。为了获得全面且可比的结果，我们以标准化方法在广泛的浓度范围内并按时间依赖性方式测试了13种FQ。当以治疗浓度使用时，所测试的FQ对人PMN吞噬或杀灭白色念珠菌以及对其活化均未产生临床上显著的影响。然而，在高浓度下，那些在N1位带有环丙基部分的FQ显示出CD11b表达增加，吞噬作用和氧化爆发减弱。
