一种用于联合测量白色念珠菌吞噬作用和细胞内杀伤的放射性测定法。
A radiometric assay for the combined measurement of phagocytosis and intracellular killing of Candida albicans.
作者信息
Bridges C G, Dasilva G L, Yamamura M, Valdimarsson H
出版信息
Clin Exp Immunol. 1980 Nov;42(2):226-33.
Abstract
A radiometric assay for combined measurement of phagocytosis and intracellular killing of Candida albicans by human polymorphonuclear leucocytes (PMNs) is presented. The assay, based upon the incorporation of 3H-uridine into the micro-organisms, makes it possible to measure phagocytosis and intracellular killing simultaneously but independently in a single sample. Thus it is possible to determine in a single assay whether increased survival of the micro-organism is due to reduced ingestion or reduced ability of the PMN to kill. The assay is objective, quantitative and convenient for clinical application. It is also suitable for analysing the effects of various agents, including serum factors and drugs, on PMN function.
摘要
本文介绍了一种用于联合测量人类多形核白细胞（PMN）对白色念珠菌的吞噬作用和细胞内杀伤作用的放射性测定法。该测定法基于将³H-尿苷掺入微生物中，使得能够在单个样本中同时但独立地测量吞噬作用和细胞内杀伤作用。因此，在一次测定中就可以确定微生物存活率的增加是由于摄取减少还是PMN杀伤能力降低。该测定法客观、定量且便于临床应用。它也适用于分析包括血清因子和药物在内的各种试剂对PMN功能的影响。
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