血浆中丙硫氧嘧啶的选择性液相色谱测定法。

Selective liquid chromatographic assay for propylthiouracil in plasma.

作者信息

Cannell G R, Williams J P, Yap A S, Mortimer R H

机构信息

Conjoint Internal Medicine Laboratory, Royal Brisbane Hospital, Queensland, Australia.

出版信息

J Chromatogr. 1991 Mar 8;564(1):310-4. doi: 10.1016/0378-4347(91)80096-u.

DOI:10.1016/0378-4347(91)80096-u

PMID:1860927

Abstract

A liquid chromatographic assay was developed to quantitate propylthiouracil in plasma using an internal standard, 5-propyl-2-thiouracil, of similar structure and physical properties. Caffeine, which coelutes with propylthiouracil, was removed by extraction from serum treated with base. No other compounds were found to interfere in the assay. The drug was extracted from plasma with chloroform with a recovery of 59.4% and the intra- and inter-assay coefficients of variation were 5.7 and 3.3%, respectively. The assay was linear to 3 micrograms/ml with a lower detection limit of 40 ng/ml for a sample volume of 1 ml.

摘要

开发了一种液相色谱分析法，使用结构和物理性质相似的内标物5-丙基-2-硫脲嘧啶来定量血浆中的丙硫氧嘧啶。与丙硫氧嘧啶共洗脱的咖啡因通过用碱处理血清后萃取去除。未发现其他化合物干扰该分析。用氯仿从血浆中萃取该药物，回收率为59.4%，分析内和分析间变异系数分别为5.7%和3.3%。对于1 ml的样品体积，该分析在3微克/毫升范围内呈线性，检测下限为40纳克/毫升。