Institute of Chemical Engineering Science and Technology, Genoa University, Via Opera Pia 15, 16145 Genova, Italy.
Biotechnol Bioeng. 1993 Mar 25;41(7):693-9. doi: 10.1002/bit.260410703.
The purpose of this study is to investigate the feasibility of biologically removing phenol from waste gases by means of a biofilter using a Pseudomonas putida strain. Two series of both batch and continuous tests have been performed in order to ascertain the microbial degradation of phenol. For the preliminary batch tests, carried out in order to test the effective feasibility of the process and to investigate their kinetic behavior, two different microbial cultures belonging to the Pseudomonas genus have been employed, a heterogeneous culture and a pure strain of P. putida. The results of these comparative investigation showed that the pure culture is more efficient than the mixed one, even when the latter has undergone three successive acclimatization tests. The continuous experiments have been conducted during a period of about 1 year in a laboratory-scale column, packed with a mixture of peat and glass beads, and utilizing the pure culture of P. putida as microflora and varying the inlet phenol concentration from 50 up to 2000 mg m(-3). The results obtained show that high degrees of conversion can be obtained (0.93/0.996) operating at a residence time of 54 s.
本研究旨在通过使用恶臭假单胞菌(Pseudomonas putida)菌株的生物过滤器来研究从废气中生物去除苯酚的可行性。为了确定苯酚的微生物降解,进行了两系列分批和连续试验。对于初步的分批试验,为了测试该过程的有效可行性并研究其动力学行为,使用了两种不同的属于假单胞菌属的微生物培养物,即异质培养物和纯恶臭假单胞菌(P. putida)菌株。这些比较研究的结果表明,即使后者已经经过了三次连续的驯化试验,纯培养物也比混合培养物更有效。连续实验在实验室规模的柱状物中进行了约一年的时间,该柱状物用泥炭和玻璃珠的混合物填充,并利用恶臭假单胞菌的纯培养物作为微生物群落,入口苯酚浓度从 50 到 2000mg m(-3)不等。所得结果表明,在停留时间为 54s 时,可以获得高转化率(0.93/0.996)。