Zhai Jian, Wang Zhu, Shi Peng, Long Chao
State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, PR China.
Department of Applied Chemistry, Nanjing Polytechnic Institute, Nanjing, PR China.
PLoS One. 2017 Jan 23;12(1):e0170417. doi: 10.1371/journal.pone.0170417. eCollection 2017.
To improve biofilter performance, the microbial community of a biofilter must be clearly defined. In this study, the performance of a lab-scale polyurethane biofilter for treating waste gas with low loads of nitrobenzene (NB) (< 20 g m-3 h-1) was investigated when using different empty bed residence times (EBRT) (64, 55.4 and 34 s, respectively). In addition, the variations of the bacterial community in the biofilm on the longitudinal distribution of the biofilters were analysed by using Illumina MiSeq high-throughput sequencing. The results showed that NB waste gas was successfully degraded in the biofilter. High-throughput sequencing data suggested that the phylum Actinobacteria and genus Rhodococcus played important roles in the degradation of NB. The variations of the microbial community were attributed to the different intermediate degradation products of NB in each layer. The strains identified in this study were potential candidates for purifying waste gas effluents containing NB.
为提高生物滤池性能，必须明确生物滤池的微生物群落。本研究考察了实验室规模的聚氨酯生物滤池在处理低负荷硝基苯（NB）（<20 g m-3 h-1）废气时，分别采用不同空床停留时间（EBRT）（分别为64、55.4和34 s）的性能。此外，利用Illumina MiSeq高通量测序分析了生物滤池纵向分布上生物膜中细菌群落的变化。结果表明，NB废气在生物滤池中被成功降解。高通量测序数据表明，放线菌门和红球菌属在NB降解中起重要作用。微生物群落的变化归因于各层中NB不同的中间降解产物。本研究中鉴定出的菌株是净化含NB废气排放物的潜在候选菌株。
