Department of Chemical Engineering, State University of New York at Buffalo, Buffalo, New York 14260-4200, USA.
Biotechnol Bioeng. 1993 Sep 20;42(7):821-8. doi: 10.1002/bit.260420706.
Microporous polymer supports for the immobilization of lipase have been prepared by the polymerization of a concentrated emulsion precursor. The concentrated emulsion consists of a mixture of styrene and divinyl-benzene containing a suitable surfactant and an initiator as the continuous phase and water as the dispersed phase. The volume fraction of the latter phase was greater than 0.74, which is the volume fraction of the dispersed phase for the most compact arrangement of spheres of equal radius. The lipase from Candida rugosa has been immobilized on the internal surface of the hydrophobic microporous poly(styrene-divinyl benzene) supports and used as biocatalysts for the hydrolysis of triacylglycerides. The effects of the amount of surfactant, of the molar ratio of divinylbenzene/styrene in the continuous phase, and of the aquaphilicity of the supports on the adsorption, activity, and stability of the immobilized lipase have been investigated. The microporous poly(styrene-divinylbenzene) adsorbents constitute excellent supports for lipase because both the amount adsorbed is large and the rate of enzymatic reaction per molecule of lipase is higher for the immobilized enzyme than for the free one.
已通过聚合浓缩乳液前体来制备用于固定化脂肪酶的微孔聚合物载体。该浓缩乳液由含有合适表面活性剂和引发剂的苯乙烯和二乙烯基苯混合物作为连续相和水作为分散相组成。后者的体积分数大于 0.74，这是等半径球体最紧密排列的分散相体积分数。来自 Rugosa 假丝酵母的脂肪酶已固定在疏水性微孔聚苯乙烯-二乙烯基苯载体的内表面上，并用作三酰基甘油水解的生物催化剂。考察了表面活性剂的量、连续相中二乙烯基苯/苯乙烯的摩尔比以及载体的亲水性对固定化脂肪酶的吸附、活性和稳定性的影响。微孔聚苯乙烯-二乙烯基苯吸附剂是脂肪酶的优良载体，因为固定化酶的吸附量较大，且每分子脂肪酶的酶反应速率高于游离酶。
