SERC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.
Biotechnol Bioeng. 1993 Oct;42(8):987-93. doi: 10.1002/bit.260420809.
Disruption of animal cells in turbulent capillary flows has been predicted from a model of cell-hydrodynamic interactions using cell mechanical properties determined by micromanipulation. Eddies of sizes similar to or smaller than the cells are presumed to interact with those cells, causing local surface deformations. The proposed mechanism of cell damage is that such deformations result in an increase in membrane tension and surface energy and that a cell disrupts when its bursting membrane tension and bursting surface energy are exceeded. The surface energy of the cells is estimated from the kinetic energy of appropriately sized eddies. To test the model, cells were disrupted in turbulent flows in capillaries at mean energy dissipation rates up to 2 x 10(4) m(2)/s(3). In all cases the model underestimated the cell disruption by about 15%. Such good agreement implies that the approach of the model to the complicated phenomena of cell turbulence interactions is reasonable.
从使用微操作确定的细胞流体力相互作用模型预测了在湍流毛细血管流中破坏动物细胞。假定大小与细胞相似或小于细胞的漩涡与这些细胞相互作用，导致局部表面变形。细胞损伤的拟议机制是，这种变形导致膜张力和表面能增加，并且当超过细胞的破裂膜张力和破裂表面能时，细胞会破裂。从适当大小的漩涡的动能估算细胞的表面能。为了测试该模型，在平均能量耗散率高达 2 x 10(4) m(2)/s(3)的情况下，在毛细血管中破坏了湍流中的细胞。在所有情况下，模型都低估了大约 15%的细胞破坏。如此良好的一致性意味着该模型接近细胞湍流相互作用的复杂现象是合理的。
