Lee J W, Cha D K, Kim I, Son A, Ahn K H
Department of Environmental Engineering, Korea University, ChoongNam 339-800, Korea.
Environ Technol. 2008 Feb;29(2):199-206. doi: 10.1080/09593330802028808.
Fatty acid methyl ester (FAME) technology was evaluated as a monitoring tool for quantification of Gordonia amarae in activated sludge systems. The fatty acid, 19:1 alcohol, which was identified as a unique fatty acid in G. amarae was not only confirmed to be present in foaming plant samples, but the quantity of the signature peak correlated closely with the degree of foaming. Foaming potential experiment provided a range of critical foaming levels that corresponded to G. amarae population. This range of critical Gordonia levels was correlated to the threshold signature FAME amount. Six full-scale wastewater treatment plants were selected based on a survey to participate in our full-scale study to evaluate the potential application of the FAME technique as the Gordonia monitoring tool. Greater amounts of signature FAME were extracted from the mixed liquor samples obtained from treatment plants experiencing Gordonia foaming problems. The amounts of signature FAME correlated well with the conventional filamentous counting technique. These results demonstrated that the relative abundance of the signature FAMEs can be used to quantitatively monitor the abundance of foam-causing microorganism in activated sludge.
脂肪酸甲酯（FAME）技术被评估为一种用于定量活性污泥系统中阿马氏戈登氏菌的监测工具。被鉴定为阿马氏戈登氏菌独特脂肪酸的19:1醇，不仅在发生泡沫的工厂样品中得到证实，而且特征峰的数量与泡沫程度密切相关。发泡潜力实验提供了一系列与阿马氏戈登氏菌数量相对应的临界发泡水平。这一系列临界戈登氏菌水平与特征FAME的阈值量相关。根据一项调查，选择了六个全尺寸污水处理厂参与我们的全尺寸研究，以评估FAME技术作为戈登氏菌监测工具的潜在应用。从经历戈登氏菌发泡问题的处理厂获得的混合液样品中提取出了更多的特征FAME。特征FAME的量与传统的丝状菌计数技术相关性良好。这些结果表明，特征FAME的相对丰度可用于定量监测活性污泥中引起泡沫的微生物的丰度。
