Park H R, Min S K, Min K, Jun S Y, Seo J, Kim H J
Department of Pathology, College of Medicine, Hallym University Sacred Heart Hospital, 896 Pyungchon-Dong, Anyang, Korea.
Acta Otolaryngol. 2009 Mar;129(3):268-72. doi: 10.1080/00016480802251591.
This study showed increased expression of p63 and survivin in cholesteatoma. Our finding indicates a putative role of p63 and survivin in the development of certain cholesteatomas.
Keratinocytes in cholesteatoma demonstrate uncoordinated hyperproliferation, migration, and invasion properties. p63 is a p53 homologue and a marker expressed in replicating keratinocytes. Survivin is an inhibitor of apoptosis protein that is abundantly expressed in most solid and hematologic malignancies. The purpose of this study was to investigate the differential expression of p63 and survivin in human middle ear cholesteatoma epithelium.
The expression levels of p63 and survivin protein were examined by immunohistochemical analysis of 40 cholesteatomas and 5 skin tissues obtained from patients during ear surgery.
Expression of p63 protein was diffusely observed in entire samples of cholesteatoma, especially in acquired cholesteatoma, compared with the control group. Congenital cholesteatoma showed variable p63 reactivity in a basal cell-like pattern. Primary and recurrent cholesteatomas showed no significant difference in p63 expression. Survivin was detected in 31 of 40 cholesteatoma samples. Acquired cholesteatomas showed especially increased survivin expression compared with congenital cases. The expression of p63 was correlated with survivin expression.
本研究显示胆脂瘤中p63和生存素的表达增加。我们的发现表明p63和生存素在某些胆脂瘤的发生发展中可能起作用。
胆脂瘤中的角质形成细胞表现出不协调的过度增殖、迁移和侵袭特性。p63是一种p53同源物，是在复制的角质形成细胞中表达的标志物。生存素是一种凋亡抑制蛋白，在大多数实体瘤和血液系统恶性肿瘤中大量表达。本研究的目的是调查p63和生存素在人中耳胆脂瘤上皮中的差异表达。
通过免疫组织化学分析对40例胆脂瘤和5例耳部手术患者的皮肤组织进行p63和生存素蛋白表达水平检测。
与对照组相比，在胆脂瘤的整个样本中均广泛观察到p63蛋白的表达，尤其是在后天性胆脂瘤中。先天性胆脂瘤在基底细胞样模式中表现出可变的p63反应性。原发性和复发性胆脂瘤在p63表达上无显著差异。在40例胆脂瘤样本中有31例检测到生存素。与先天性病例相比，后天性胆脂瘤中生存素表达尤其增加。p63的表达与生存素的表达相关。
