Izutsu K, Yoshioka S, Terao T
National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, 158 Tokyo, Japan.
Biotechnol Bioeng. 1994 May;43(11):1102-7. doi: 10.1002/bit.260431114.
The effects of amphiphilic excipients on the inactivation of lactate dehydrogenase (LDH) during freeze-thawing and freeze-drying were studied. Some amphiphilic excipients such as hydroxypropyl-beta-cyclodextrin (HP-beta-CD), CHAPS, polyethylene glycol (PEG) 3350, and sucrose fatty acid monoester prevented LDH inactivation during freeze-thawing and freeze-drying at a lower concentration than sugars and amino acids. Polyoxyethylene 9 lauryl ether and PEG 400 protected LDH during freeze-thawing but not during freeze-drying. The buffer concentration of the solution to be freeze-dried (10, 50, and 200 mM) affected the stabilizing effect of trehalose, but not that of HP-beta-CD. (c) 1994 John Wiley & Sons, Inc.
研究了两亲性辅料在冻融和冻干过程中对乳酸脱氢酶（LDH）失活的影响。一些两亲性辅料，如羟丙基-β-环糊精（HP-β-CD）、CHAPS、聚乙二醇（PEG）3350和蔗糖脂肪酸单酯，在冻融和冻干过程中以比糖类和氨基酸更低的浓度防止LDH失活。聚氧乙烯9月桂基醚和PEG 400在冻融过程中保护LDH，但在冻干过程中则不然。待冻干溶液的缓冲液浓度（10、50和200 mM）影响海藻糖的稳定作用，但不影响HP-β-CD的稳定作用。（c）1994约翰·威利父子公司。
