Xiao Xinglong, Zhang Jingwei, Gong Jun, Pan Yanping, Yu Yigang, Yang Xiaoquan, Wu Hui
College of Light Industry & Food, South China University of Technology, Guangzhou 510640, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Apr;24(4):581-5. doi: 10.1016/s1872-2075(08)60029-1.
Pseudomonas aernginosa (PA) is one of the most universal pathogens in clinical diagnosis, and conventional detection assay has many disadvantages. In this research, a pair of specific primers and a TaqMan fluorescent probe were designed in the conservative region of ETA gene by the method of bioinformatics analysis, the detection method for PA was successfully developed. Different gradient concentrations of PA DNA and various pathogen DNA were amplified by fluorescence quantitative PCR (FQ-PCR) to confirm the specificity and sensitivity of the developed method. Results showed that the developed detection assay is more sensible and specific by comparison to the conventional FQ-PCR method, and it is valuable for research and application prospects.
铜绿假单胞菌（PA）是临床诊断中最常见的病原体之一，传统检测方法存在诸多缺点。本研究通过生物信息学分析方法，在ETA基因保守区设计了一对特异性引物和一条TaqMan荧光探针，成功建立了PA的检测方法。通过荧光定量PCR（FQ-PCR）对不同梯度浓度的PA DNA和多种病原体DNA进行扩增，以确认所建立方法的特异性和灵敏度。结果表明，与传统FQ-PCR方法相比，所建立的检测方法更灵敏、特异，具有一定的研究价值和应用前景。
