[在重组毕赤酵母中高效生产碱性聚半乳糖醛酸裂解酶]

[High-level production of alkaline polygalacturonate lyase in recombinant Pichia pastoris].

作者信息

Wang Yun, Hua Zhaozhe, Liu Liming, Zhang Zhaohui, Du Guocheng, Chen Jian

机构信息

Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2008 Apr;24(4):635-9.

PMID:18616175

Abstract

In order to increase the production of alkaline polygalacturonate lyase (PGL) by recombinant Pichia pastoris GS115, the effect of cell and methanol concentration on the PGL production was carefully investigated by single factor experiment. The optimum conditions were listed as follows: the cell concentration 122 g/L, the methanol concentration 20 g/L, and the ratio of methanol and cell concentration 0.16-0.20 g/g (methanol/cell). With the glycerol and methanol feeding strategies, the ratio of methanol and cell concentration could be controlled at the range of 0.171 to 0.195 g/g. And the highest PGL activity (430 u/mL) and highest PGL productivity (4.34 u/mL/h) were achieved.

摘要

为提高重组毕赤酵母GS115产碱性聚半乳糖醛酸裂解酶（PGL）的能力，通过单因素实验仔细研究了细胞浓度和甲醇浓度对PGL产量的影响。最佳条件如下：细胞浓度122 g/L，甲醇浓度20 g/L，甲醇与细胞浓度之比为0.16 - 0.20 g/g（甲醇/细胞）。采用甘油和甲醇补料策略，甲醇与细胞浓度之比可控制在0.171至0.195 g/g范围内。并且实现了最高的PGL活性（430 u/mL）和最高的PGL生产效率（4.34 u/mL/h）。