[混合碳源对重组毕赤酵母发酵生产聚半乳糖醛酸裂解酶的影响]

[Effects of mixed carbon sources in cultivation of recombinant Pichia pastoris for polygalacturonate lyase production].

作者信息

Wang Zhihao, Zhang Dongxu, Li Jianghua, Du Guocheng, Chen Jian

机构信息

Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2009 Dec;25(12):1955-61.

PMID:20352974

Abstract

In order to increase the production and productivity of alkaline polygalacturonate lyase (PGL), we studied the mixed carbon sources feeding strategies during the induction phase by recombinant Pichia pastoris GS 115. Glycerol, sorbitol or lactic acid co-feeding with methanol all enhanced the PGL production. Among all the feeding strategies, the sorbitol co-feeding strategy was most significant. By using this strategy, the PGL activity and productivity reached 1593 U/mL and 16.7 U/(mL-h). Compared to the control, the enhancements of PGL activity and productivity were 84.6% and 45.2% respectively, when we set the sorbitol feeding rate at 3.6 g/(h x L).

摘要

为了提高碱性聚半乳糖醛酸裂解酶（PGL）的产量和生产效率，我们研究了重组毕赤酵母GS 115在诱导阶段的混合碳源补料策略。甘油、山梨醇或乳酸与甲醇共同补料均提高了PGL的产量。在所有补料策略中，山梨醇共同补料策略最为显著。采用该策略时，PGL活性和生产效率分别达到1593 U/mL和16.7 U/(mL·h)。当我们将山梨醇补料速率设定为3.6 g/(h·L)时，与对照相比，PGL活性和生产效率分别提高了84.6%和45.2%。