Djogbénou L, Akogbéto M, Chandre F
Centre de Recherche Entomologique de Cotonou, Cotonou, Benin.
Acta Trop. 2008 Sep;107(3):272-4. doi: 10.1016/j.actatropica.2008.06.004. Epub 2008 Jun 21.
Resistance to organophosphates and carbamates was investigated in three samples of Culex pipiens quinquefasciatus Say, from Cotonou and Parakou in the Republic of Benin. Each population of larvae was selected with propoxur to eliminate susceptible individuals and more easily analyse resistant mosquitoes if the resistance allele is present in the field sample. The mass-selection showed that there are resistant mosquitoes in natural populations of C. p. quinquefasciatus in Benin and that nowadays they are still at a low frequency. The result of the PCR-based assay revealed the presence of the G119S mutation in all the C. p. quinquefasciatus mosquitoes that survived exposure to mass-selection. This mutation is the same than reported in a large range of insects, including Anopheles gambiae. Thus, the G119S mutation is spreading in natural populations of C. p. quinquefasciatus in Africa. Therefore, good mosquito control strategies and agricultural use of insecticides are critical, and should be carefully applied to limit the spread of this resistance gene.
对来自贝宁共和国科托努和帕拉库的三批致倦库蚊样本进行了有机磷和氨基甲酸酯抗性研究。对每批幼虫群体用残杀威进行筛选，以清除敏感个体，从而在野外样本中存在抗性等位基因时更容易分析抗性蚊子。大规模筛选表明，贝宁的致倦库蚊自然种群中存在抗性蚊子，且目前其频率仍然较低。基于聚合酶链式反应（PCR）检测的结果显示，所有在大规模筛选中存活下来的致倦库蚊都存在G119S突变。这种突变与在包括冈比亚按蚊在内的大量昆虫中报道的相同。因此，G119S突变正在非洲致倦库蚊的自然种群中传播。所以，良好的蚊虫控制策略和杀虫剂在农业上的使用至关重要，应谨慎应用以限制这种抗性基因的传播。
