Choi Soo-Youn, Hwang Joon-Ho, Park Soo-Young, Jin Yeong-Jun, Ko Hee-Chul, Moon Sang-Wook, Kim Se-Jae
Technology Innovation Center for Life Science, Cheju National University, Jeju, 690-756, South Korea.
Phytother Res. 2008 Aug;22(8):1030-4. doi: 10.1002/ptr.2419.
The goal of this study was to elucidate the antiinflammatory activities of Psidium guajava L. (guava) leaf. To improve the functionality of guava leaf, it was fermented with Phellinus linteus mycelia, Lactobacillus plantarum and Saccharomyces cerevisiae. The ethanol extract from fermented guava leaf inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E(2) (PGE(2)) production. Western blot analysis showed that fermented guava leaf extract decreased LPS-induced inducible nitric oxide synthase (iNOS) and the cyclooxygenase-2 (COX-2) protein level in RAW 264.7 cells. To investigate the mechanism involved, the study examined the effect of fermented guava leaf extract on LPS-induced nuclear factor-kappaB (NF-kappaB) activation. Fermented guava leaf extract significantly inhibited LPS-induced NF-kappaB transcriptional activity. Immunochemical analysis revealed that fermented guava leaf extract suppressed LPS-induced degradation of I-kappaBalpha. Taken together, the data indicate that fermented guava leaf extract is involved in the inhibition of iNOS and COX-2 via the down-regulation of NF-kappaB pathway, revealing a partial molecular basis for the antiinflammatory properties of fermented guava leaf extract.
本研究的目的是阐明番石榴叶的抗炎活性。为提高番石榴叶的功能特性，将其与桑黄菌丝体、植物乳杆菌和酿酒酵母进行发酵。发酵番石榴叶的乙醇提取物可抑制脂多糖（LPS）诱导的一氧化氮（NO）和前列腺素E2（PGE2）的产生。蛋白质印迹分析表明，发酵番石榴叶提取物可降低LPS诱导的RAW 264.7细胞中诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）的蛋白水平。为探究其中涉及的机制，本研究检测了发酵番石榴叶提取物对LPS诱导的核因子-κB（NF-κB）激活的影响。发酵番石榴叶提取物显著抑制LPS诱导的NF-κB转录活性。免疫化学分析显示，发酵番石榴叶提取物可抑制LPS诱导的I-κBα降解。综上所述，数据表明发酵番石榴叶提取物通过下调NF-κB途径参与对iNOS和COX-2的抑制，揭示了发酵番石榴叶提取物抗炎特性的部分分子基础。
