Shimotohno K, Miura K
National Institute of Genetics, Mishima, 411 Japan.
Virology. 1973 May;53(1):283-6.
RNA polymerase associated with cytoplasmic polyhedrosis virus, which contains double-stranded RNA as a genome, synthesizes single-stranded RNA in vitro. The RNA product has the same size distribution to the genome segments, showing two fractions on a glycerol concentration gradient centrifugation. Each fraction of the synthesized RNA hybridized specifically with the corresponding size fraction of the denatured genome RNA segments. Only after the transcription has been carried out over the whole length of each genome segment, the single-stranded RNA product leaves the virus particle. It seems that the transcription of every segment starts at the same time, although the transcribed RNA for the shorter segment group is released from a virion faster than the larger group.
与细胞质多角体病毒相关的RNA聚合酶，其基因组为双链RNA，可在体外合成单链RNA。RNA产物与基因组片段具有相同的大小分布，在甘油浓度梯度离心中呈现两个组分。合成的RNA的每个组分都与变性基因组RNA片段的相应大小组分特异性杂交。只有在每个基因组片段的全长都完成转录后，单链RNA产物才离开病毒颗粒。似乎每个片段的转录同时开始，尽管较短片段组的转录RNA比大片段组更快地从病毒体中释放出来。
