Department of General Zoology, University of Amsterdam, Biological Centre, Anna's Hoeve, Kruislaan 320, 1098 SM Amsterdam, The Netherlands.
Tissue Cell. 1986;18(3):469-78. doi: 10.1016/0040-8166(86)90066-2.
The transformation of the slow contracting larval m. obliquus lateralis caudalis II during metamorphosis into the asynchronous indirect flight muscle, m. obliquus lateralis dorsalis, in the Colorado beetle, Leptinotarsa decemlineata, was examined by electron microscopy. Particular attention was paid to the fate of the larval muscle fibres, the origin and behaviour of the myoblasts for flight muscle development and the change of the myofibrillar filament lattice of the larva into that of the adult. In the pre-pupal period, the larval muscles dedifferentiate and fragment. At pupation, the muscle fibres consist of cell fragments containing very few myofibrils. The sarcoplasmic reticulum and the transverse tubular system are greatly reduced. The number of myoblasts developed from satellite cells by mitosis increases considerably. They penetrate the muscle fibre and surround the cell fragments. The new fibres of the flight muscle develop from myocytes fused with the larval fragments. The larval basal lamina, surrounding the cell fragments and myoblasts, is present in pupae up to 1 day old. In pupae about 2.5 days old new myofibrils appear that have the adult filament lattice. The insect muscle transformation and the repair of vertebrate muscle after injury show striking resemblances.
幼虫尾部外侧斜肌 II 在变态过程中向异步间接飞行肌，即成虫外侧斜肌背侧肌的转化，在异色瓢虫中，被电子显微镜检查。特别关注幼虫肌肉纤维的命运、飞行肌肉发育的成肌细胞的起源和行为以及幼虫肌原纤维丝晶格向成虫的变化。在预蛹期，幼虫肌肉去分化并分裂。在化蛹时，肌肉纤维由含有很少肌原纤维的细胞碎片组成。肌浆网和横小管系统大大减少。由卫星细胞有丝分裂产生的成肌细胞数量显著增加。它们穿透肌肉纤维并包围细胞碎片。新的飞行肌纤维由与幼虫碎片融合的肌细胞发育而来。幼虫的基膜，围绕着细胞碎片和成肌细胞，在蛹中存在直到 1 天龄。在大约 2.5 天大的蛹中，出现了具有成人丝状晶格的新肌原纤维。昆虫肌肉的转化和脊椎动物肌肉受伤后的修复表现出惊人的相似之处。
