Carrascosa J L, Viñuela E, Salas M
Department of Molecular Biology, Institute G. Marañón, C.S.I.C., Velázquez, 144-Madrid 6, Spain.
Virology. 1973 Nov;56(1):291-9.
Bacteriophage phi29 induces, in UV-irradiated B. subtilis, the synthesis of 12 non-structural proteins as well as the 7 structural polypeptides which form the viral particle. The molecular weight of the nonstructural polypeptides amounts to 180,000 daltons; if all these proteins are phage-coded, this corresponds to about 30% of the information content of phi29 DNA. The phage-induced polypeptides can be classified as early and late proteins, according with the time of their appearance after infection. All the structural proteins as well as three nonstructural proteins are late proteins. The remaining nonstructural proteins appear early after infection.
噬菌体φ29在紫外线照射的枯草芽孢杆菌中诱导合成12种非结构蛋白以及构成病毒粒子的7种结构多肽。非结构多肽的分子量为180,000道尔顿;如果所有这些蛋白都是由噬菌体编码的,这大约相当于φ29 DNA信息含量的30%。根据噬菌体诱导的多肽在感染后出现的时间,可将其分为早期蛋白和晚期蛋白。所有结构蛋白以及三种非结构蛋白都是晚期蛋白。其余非结构蛋白在感染后早期出现。