Huang Yu-Chuen, Chen Marcelo, Shyr Yi-Ming, Su Cheng-Hsi, Chen Chi-Kuan, Li Anna Fen-Yau, Ho Donald Ming-Tak, Chen Yi-Ming Arthur
AIDS Prevention and Research Center, National Yang-Ming University, Taipei, Taiwan.
J Gastroenterol Hepatol. 2008 Sep;23(9):1384-9. doi: 10.1111/j.1440-1746.2008.05488.x. Epub 2008 Jul 8.
Glycine N-methyltransferase (GNMT) is a susceptibility gene for human hepatocellular carcinoma (HCC). We previously reported that GNMT expression is diminished in HCC. Here we report our examination of GNMT expression patterns in cholangiocarcinoma and the relationship between its expression and prognosis.
We analyzed GNMT expression in tumor tissues from 33 cholangiocarcinoma patients (19 male) using immunohistochemistry (IHC) procedures with a GNMT monoclonal antibody (mAb 4-17). GNMT expression intensity and percentages were scored on a scale of 0 to 6. The association between GNMT expression and survival was analyzed using the Kaplan-Meier method, and prognostic factors were evaluated with a multivariate Cox proportional hazards regression model.
High GNMT expression was found in epithelial cells of normal bile ducts. Six of 33 (18.2%) cholangiocarcinoma tissues had no GNMT expression. A statistically significant difference was noted in GNMT expression between male and female patients (68.4% vs 100%, P < 0.05). Compared to patients with GNMT expression scores > 3, the death hazard ratio for patients with GNMT scores <or= 3 was 3.68 (95% confidence interval = 1.17-11.59, P < 0.05).
GNMT expression is a favorable prognosis predictor for cholangiocarcinoma.
甘氨酸N-甲基转移酶（GNMT）是人类肝细胞癌（HCC）的一个易感基因。我们之前报道过GNMT在HCC中的表达降低。在此，我们报告我们对胆管癌中GNMT表达模式及其表达与预后关系的研究。
我们使用GNMT单克隆抗体（mAb 4-17），通过免疫组织化学（IHC）方法分析了33例胆管癌患者（19例男性）肿瘤组织中的GNMT表达。GNMT表达强度和百分比按0至6分进行评分。使用Kaplan-Meier方法分析GNMT表达与生存之间的关联，并使用多变量Cox比例风险回归模型评估预后因素。
在正常胆管上皮细胞中发现高GNMT表达。33例胆管癌组织中有6例（18.2%）无GNMT表达。男性和女性患者的GNMT表达存在统计学显著差异（68.4%对100%，P < 0.05）。与GNMT表达评分> 3的患者相比，GNMT评分≤3的患者死亡风险比为3.68（95%置信区间 = 1.17 - 11.59，P < 0.05）。
GNMT表达是胆管癌预后的一个有利预测指标。
