一种与毒蕈碱型乙酰胆碱受体结合的蛇毒毒素的氨基酸序列。

Amino acid sequence of a snake venom toxin that binds to the muscarinic acetylcholine receptor.

作者信息

Karlsson E, Risinger C, Jolkkonen M, Wernstedt C, Adem A

机构信息

Department of Biochemistry, Biomedical Centre, Uppsala, Sweden.

出版信息

Toxicon. 1991;29(4-5):521-6. doi: 10.1016/0041-0101(91)90027-o.

DOI:10.1016/0041-0101(91)90027-o

PMID:1862525

Abstract

The green mamba, Dendroaspis angusticeps, has two protein toxins that bind to the muscarinic acetylcholine receptor. The sequence of muscarinic toxin 2 was determined with an automatic gas phase sequencer. The C-terminal residue is Asp as determined by hydrazinolysis and amino acid analysis. Toxin 2 has 65 amino acid residues and a formula weight of 7040. It is homologous to a large number of other snake venom toxins as short alpha-neurotoxins, cardiotoxins/cytotoxins and angusticeps-type toxins of mamba venoms. The sequence is confirmed in the accompanying article (Ducancel, F., Rowan, E.G., Cassart, E., Harvey, A. L., Menez, A. and Boulain, J.-C. Toxicon 29, 516-520, 1991).

摘要

绿曼巴蛇（Dendroaspis angusticeps）有两种能与毒蕈碱型乙酰胆碱受体结合的蛋白质毒素。用自动气相测序仪测定了毒蕈碱毒素2的序列。通过肼解和氨基酸分析确定其C末端残基为天冬氨酸。毒素2有65个氨基酸残基，分子量为7040。它与大量其他蛇毒毒素同源，如短α-神经毒素、心脏毒素/细胞毒素以及曼巴蛇毒的竹叶青型毒素。该序列在随附文章中得到证实（Ducancel, F., Rowan, E.G., Cassart, E., Harvey, A. L., Menez, A. 和Boulain, J.-C. 《毒素学》29, 516 - 520, 1991）。