Vihinen Pia, Koskivuo Ilkka, Syrjänen Kari, Tervahartiala Taina, Sorsa Timo, Pyrhönen Seppo
Departments of aOncology and Radiotherapy bSurgery, Turku University Hospital, Turku, Finland.
Melanoma Res. 2008 Aug;18(4):268-73. doi: 10.1097/CMR.0b013e3283090031.
Serological markers of malignant melanoma have failed to provide prognostic significance in patients who are tumour-free after surgery. Immune response regulation is important regarding progression and therapeutic interventions of malignant melanoma. Matrix metalloproteinase (MMP)-8 is one of the collagenases involved in the regulation of tissue remodelling and immune response, being incompletely studied in melanoma as yet. We assessed whether serum MMP-8 is of prognostic value in malignant melanoma. We studied serum samples of 117 patients, of which 63 were stage I, 13 stage II, 12 stage III and 29 stage IV. The mean serum MMP-8 levels (47.5 ng/ml) did not significantly correlate with patient or tumour characteristics, that is, patient sex, age, tumour Clark's or Breslow's classification, sentinel lymph node status or to survival. Importantly, high serum MMP-8 levels were significantly related to presence of vascular invasion (P=0.001) in primary tumour, tumour ulceration (P=0.003) and tumour bleeding (P=0.033). Tendency to increased serum MMP-8 levels in patients with coronary heart disease or type II diabetes mellitus was detected. These data imply that high serum MMP-8 level is associated with earlier recognized histopathology markers of melanoma progression. Results also suggest that elevated serum MMP-8 might be related to haematogenous spreading of melanoma through vascular invasion.
恶性黑色素瘤的血清学标志物在术后无肿瘤的患者中未能提供预后意义。免疫反应调节对于恶性黑色素瘤的进展和治疗干预很重要。基质金属蛋白酶（MMP）-8是参与组织重塑和免疫反应调节的胶原酶之一，在黑色素瘤中尚未得到充分研究。我们评估了血清MMP-8在恶性黑色素瘤中是否具有预后价值。我们研究了117例患者的血清样本，其中63例为I期，13例为II期，12例为III期，29例为IV期。血清MMP-8的平均水平（47.5 ng/ml）与患者或肿瘤特征，即患者性别、年龄、肿瘤Clark或Breslow分类、前哨淋巴结状态或生存率，均无显著相关性。重要的是，血清MMP-8水平高与原发性肿瘤中的血管侵犯（P=0.001）、肿瘤溃疡（P=0.003）和肿瘤出血（P=0.033）显著相关。在冠心病或II型糖尿病患者中检测到血清MMP-8水平有升高趋势。这些数据表明，血清MMP-8水平高与黑色素瘤进展的早期组织病理学标志物相关。结果还表明，血清MMP-8升高可能与黑色素瘤通过血管侵犯的血行播散有关。
