Nahorski S R, Smith B M
Eur J Pharmacol. 1976 Dec;40(2):273-8. doi: 10.1016/0014-2999(76)90062-5.
Marked accumulation of cerebral cyclic AMP was induced in the chick by the i.v. administration of isoprenaline or histamine of by decapitation. These responses were examined in various cerebral regions and compared to the responses produced in vitro when maximal concentrations of isoprenaline, histamine or adenosine were added to appropriate brain slices. Histamine only markedly stimulated cyclic AMP formation in the cerebral hemispheres. Isoprenaline was equally potent in the cerebellum and the cerebral hemispheres though the medulla-diencephalon and optic lobes were less responsive. There was a lack of correlation between the effects of decapitation in vivo and the responses to adenosine in vitro. Similar accumulations of cyclic AMP were produced by decapitation in the cerebral hemispheres and cerebellum though adenosine was much more effective on cerebellar slices in vitro. The topographical distribution of catecholamine and histamine sensitive cyclic AMP forming sites do not correlate with endogenous levels of noradrenaline or histamine in chick brain and possible interpretations are discussed.
静脉注射异丙肾上腺素或组胺，或断头处理可使雏鸡大脑中环状腺苷酸（cyclic AMP）显著蓄积。对这些反应在大脑的不同区域进行了检测，并与在体外向合适的脑切片中添加最大浓度的异丙肾上腺素、组胺或腺苷时所产生的反应进行了比较。组胺仅显著刺激大脑半球中环状腺苷酸的形成。异丙肾上腺素在小脑和大脑半球中的作用效果相同，不过延髓-间脑和视叶的反应较弱。体内断头处理的效果与体外对腺苷的反应之间缺乏相关性。断头处理在大脑半球和小脑中产生了类似的环状腺苷酸蓄积，不过腺苷在体外对小脑切片的作用更为有效。儿茶酚胺和组胺敏感的环状腺苷酸形成位点的拓扑分布与雏鸡大脑中去甲肾上腺素或组胺的内源性水平不相关，并对可能的解释进行了讨论。
