Nakata Makoto, Itou Takuya, Sakai Takeo
Nihon University Veterinary Research Center, Kanagawa, Japan.
J Vet Med Sci. 2008 Jun;70(6):543-50. doi: 10.1292/jvms.70.543.
The present study determined the cDNA and deduced amino acid sequences of ferret (Mustela putorius furo) inflammatory cytokines, interferon (IFN)-gamma, interleukin (IL)-1beta, IL-6, IL-8 and tumor necrosis factor (TNF)-alpha. The homologies of the nucleotide sequences of IFN-gamma, IL-1beta, IL-6, IL-8 and TNF-alpha of the ferret to those from other mammalian species ranged from 64.3-92.9%, 73.0-83.9, 58.1-84.8%, 58.1-89.7% and 79.0-95.0%, respectively. As distinctive amino acid residues constituting various motifs and ligand-binding sites and cysteine residues were highly conserved in ferret inflammatory cytokine proteins, ferret cytokines may have fundamentally similar functions to those of other mammals. Phylogenetic analyses based on the deduced amino acid sequences revealed that all ferret inflammatory cytokines were more closely related to those of the Carnivora order, specifically dog and cat, than to other species.
本研究确定了雪貂（鼬属雪貂）炎性细胞因子、干扰素（IFN）-γ、白细胞介素（IL）-1β、IL-6、IL-8和肿瘤坏死因子（TNF）-α的cDNA及推导的氨基酸序列。雪貂IFN-γ、IL-1β、IL-6、IL-8和TNF-α的核苷酸序列与其他哺乳动物物种相应序列的同源性分别为64.3 - 92.9%、73.0 - 83.9%、58.1 - 84.8%、58.1 - 89.7%和79.0 - 95.0%。由于构成各种基序和配体结合位点的独特氨基酸残基以及半胱氨酸残基在雪貂炎性细胞因子蛋白中高度保守，雪貂细胞因子的功能可能与其他哺乳动物的基本相似。基于推导氨基酸序列的系统发育分析表明，所有雪貂炎性细胞因子与食肉目动物（特别是狗和猫）的细胞因子相比，与其他物种的关系更为密切。
