Gong Xiaoming, Lin Tong, Sun Zhaohui, Fu Min, Zuo Huancong, Xie Zuoping
Medical college in Tsinghua University, Beijing 100084, China.
Neuroreport. 2008 Aug 6;19(12):1203-7. doi: 10.1097/WNR.0b013e328308b322.
Olig1 is a transcription factor that is essential for oligodendrogenesis. It is important to understand the upstream regulation of Olig1 expression because of its critical role in remyelination repair. A mouse oligodendrocyte progenitor cell (OPC) differentiation model was established to explore Olig1 transcriptional activity during OPC differentiation. Using an Olig1 promoter-luciferase reporter plasmid, we found that Olig1 transcription is dramatically decreased during OPC differentiation in response to 0.5% fetal bovine serum. Olig1 protein expression is also remarkably decreased as revealed by immunostaining and western blotting. Thus, Olig1 is downregulated during OPC differentiation, suggesting that Olig1 is not actively required in differentiated oligodendrocytes.
少突胶质细胞转录因子1（Olig1）是少突胶质细胞生成所必需的转录因子。鉴于其在髓鞘再生修复中的关键作用，了解Olig1表达的上游调控机制非常重要。为了探究少突胶质前体细胞（OPC）分化过程中Olig1的转录活性，建立了小鼠少突胶质前体细胞分化模型。使用Olig1启动子-荧光素酶报告质粒，我们发现，在0.5%胎牛血清刺激下，OPC分化过程中Olig1转录显著降低。免疫染色和蛋白质印迹显示，Olig1蛋白表达也显著降低。因此，OPC分化过程中Olig1表达下调，提示分化的少突胶质细胞中Olig1并非发挥积极作用所必需。
