Landegren Ulf, Dahl Fredrik, Nilsson Mats, Fredriksson Simon, Banér Johan, Gullberg Mats, Jarvius Jonas, Gustafsdottir Sigrun, Söderberg Ola, Ericsson Olle, Stenberg Johan, Schallmeiner Edith
Department of Genetics and Pathology, Rudbeck Laboratory, Uppsala University, Sweden.
Comp Funct Genomics. 2003;4(5):525-30. doi: 10.1002/cfg.326.
Highly specific high-throughput assays will be required to take full advantage of the accumulating information about the macromolecular composition of cells and tissues, in order to characterize biological systems in health and disease. We discuss the general problem of detection specificity and present the approach our group has taken, involving the reformatting of analogue biological information to digital reporter segments of genetic information via a series of DNA ligation assays. The assays enable extensive, coordinated analyses of the numbers and locations of genes, transcripts and protein.
为了全面了解健康和疾病状态下的生物系统，充分利用有关细胞和组织大分子组成的不断积累的信息，将需要高度特异性的高通量检测方法。我们讨论了检测特异性的一般问题，并介绍了我们团队所采用的方法，该方法涉及通过一系列DNA连接检测将类似物生物信息重新格式化为遗传信息的数字报告片段。这些检测能够对基因、转录本和蛋白质的数量及位置进行广泛、协调的分析。
