De Las Rivas Javier, de Luis Alberto
Cancer Research Center (CIC, USAL-CSIC), University of Salamanca and CSIC, Campus Miguel de Unamuno, Salamanca E37007, Spain.
Comp Funct Genomics. 2004;5(2):173-8. doi: 10.1002/cfg.377.
In recent years, the biomolecular sciences have been driven forward by overwhelming advances in new biotechnological high-throughput experimental methods and bioinformatic genome-wide computational methods. Such breakthroughs are producing huge amounts of new data that need to be carefully analysed to obtain correct and useful scientific knowledge. One of the fields where this advance has become more intense is the study of the network of 'protein-protein interactions', i.e. the 'interactome'. In this short review we comment on the main data and databases produced in this field in last 5 years. We also present a rationalized scheme of biological definitions that will be useful for a better understanding and interpretation of 'what a protein-protein interaction is' and 'which types of protein-protein interactions are found in a living cell'. Finally, we comment on some assignments of interactome data to defined types of protein interaction and we present a new bioinformatic tool called APIN (Agile Protein Interaction Network browser), which is in development and will be applied to browsing protein interaction databases.
近年来，新的生物技术高通量实验方法和生物信息学全基因组计算方法取得了压倒性的进展，推动了生物分子科学的发展。此类突破正在产生海量的新数据，需要对其进行仔细分析，以获取正确且有用的科学知识。蛋白质 - 蛋白质相互作用网络（即“相互作用组”）研究领域是这一进展最为显著的领域之一。在这篇简短的综述中，我们对过去五年该领域产生的主要数据和数据库进行了评论。我们还提出了一个生物学定义的合理化方案，这将有助于更好地理解和解释“什么是蛋白质 - 蛋白质相互作用”以及“在活细胞中发现了哪些类型的蛋白质 - 蛋白质相互作用”。最后，我们对将相互作用组数据分配到特定类型蛋白质相互作用的一些情况进行了评论，并展示了一种名为APIN（敏捷蛋白质相互作用网络浏览器）的新生物信息工具，该工具正在开发中，将用于浏览蛋白质相互作用数据库。
