Thomas James W
Department of Human Genetics, Emory University School of Medicine, Atlanta, GA.
Methods Mol Biol. 2008;422:119-32. doi: 10.1007/978-1-59745-581-7_8.
Comparative genomics is a powerful approach for inferring the history and function of genomic sequence. The generation of bacterial artificial chromosome (BAC)-based physical maps is a proven method for the targeted comparative genomic analysis of genes or regions of interest. 'Universal' overgo hybridization probes can be used for the efficient construction of BAC-based physical maps of orthologous chromosome segments from multiple species in parallel. 'Universal' overgo hybridization probes can therefore facilitate the assembly of deep and diverse collections of experimental and computational comparative genomic resources corresponding to specific segments of the genome. The design of 'universal' overgo probes is dependent on the presence of sequences that are highly conserved within a group of species. Such conserved sequences can be readily identified using local-or whole-genome interspecies sequence alignments. Once 'universal' overgo hybridization probes are designed, simple and uniform labeling and hybridization conditions can be carried out to exploit the utility of these probes for targeted comparative physical mapping.
比较基因组学是推断基因组序列历史和功能的一种强大方法。基于细菌人工染色体（BAC）构建物理图谱是一种经过验证的方法，可用于对感兴趣的基因或区域进行靶向比较基因组分析。“通用”重叠杂交探针可用于并行高效构建多个物种直系同源染色体片段的基于BAC的物理图谱。因此，“通用”重叠杂交探针有助于组装与基因组特定片段相对应的丰富多样的实验和计算比较基因组资源。“通用”重叠杂交探针的设计取决于一组物种中高度保守的序列的存在。使用局部或全基因组种间序列比对可以很容易地识别出这些保守序列。一旦设计出“通用”重叠杂交探针，就可以采用简单统一的标记和杂交条件，以利用这些探针进行靶向比较物理作图。
