Waters Paul D, Marshall Graves Jennifer A, Thompson Katherine, Sankovic Natasha, Ezaz Tariq
Research School of Biological Sciences, The Australian National University, Canberra, Australia.
Methods Mol Biol. 2008;422:239-51. doi: 10.1007/978-1-59745-581-7_15.
Comparative molecular cytogenetics provides a powerful tool for deciphering the evolutionary history of vertebrate sex chromosomes. We have adapted cell culture and molecular cytogenetic techniques to study the sex chromosomes of many exotic mammals, birds, and reptiles. Here we describe differential chromosome banding and staining techniques that distinguish sex chromosomes in species with no morphologically distinct XY or ZW chromosome pairs. We describe a method to isolate, identify, and map genomic BAC clones from the Y chromosome, and we also identify strategies for isolating candidate sex chromosome genes.
比较分子细胞遗传学为解读脊椎动物性染色体的进化史提供了一个强大的工具。我们采用细胞培养和分子细胞遗传学技术来研究多种外来哺乳动物、鸟类和爬行动物的性染色体。在此，我们描述了可区分那些没有形态上明显不同的XY或ZW染色体对的物种中性染色体的差异染色体显带和染色技术。我们描述了一种从Y染色体分离、鉴定和定位基因组BAC克隆的方法，并且我们还确定了分离候选性染色体基因的策略。
