Sannier F, Lecoeur C, Zhao Q, Garreau I, Piot J M
Laboratoire de Génie Protéique, Pôle des Sciences et Technologies Université de La Rochelle, Av. Marillac, 17042, La Rochelle Cédex 1, France.
Biotechnol Bioeng. 1996 Nov 20;52(4):501-6. doi: 10.1002/(SICI)1097-0290(19961120)52:4<501::AID-BIT5>3.0.CO;2-T.
Efficiency and selectivity of 30 and 150 kd inorganic ultrafiltration membranes (Techsep) toward tuna hemoglobin and myoglobin were studied. The influence of pH and ionic strength was investigated. Mass flow of myoglobin was higher at its isoelectric pH (8.6) and for low ionic strength (1.5 mM). This result was related to the absence of electrostatic repulsion between myoglobin and the surface of the dynamic membrane. The use of high ionic strength 0.15 M NaCl involved an apparent dimerisation of myoglobin and consequently a lower permeation through the membrane due to the molecular weight increase. The permeation and retention of hemoglobin did not agree with the effect of pH observed with myoglobin (best permeation at isoelectric pH) but followed the behavior of myoglobin. This was explained by a myoglobin concentration 10 times higher than hemoglobin concentration. The yield of retention selectivity was investigated. Selectivity of the membrane at pH 8.6 and 1.5 mM was favorable to myoglobin (increase of 40%) whereas a reversed selectivity was observed at pH 7.3, 0.15 M. (c) 1996 John Wiley & Sons, Inc.
研究了30kd和150kd无机超滤膜（Techsep）对金枪鱼血红蛋白和肌红蛋白的效率和选择性。研究了pH值和离子强度的影响。肌红蛋白在其等电点pH值（8.6）和低离子强度（1.5 mM）下的质量流量较高。该结果与肌红蛋白和动态膜表面之间不存在静电排斥有关。使用高离子强度的0.15M NaCl会导致肌红蛋白明显二聚化，因此由于分子量增加，膜的渗透通量降低。血红蛋白的渗透和截留情况与肌红蛋白观察到的pH值影响不一致（在等电点pH值时渗透最佳），但遵循肌红蛋白的行为。这是由于肌红蛋白浓度比血红蛋白浓度高10倍所致。研究了截留选择性的产率。膜在pH 8.6和1.5 mM时对肌红蛋白具有选择性（增加40%），而在pH 7.3、0.15M时观察到相反的选择性。(c) 1996约翰威立父子公司。
