Emel'ianov V I, Kravchuk Zh N, Poliakovskiĭ S A, Dmitriev A P
Tsitol Genet. 2008 Mar-Apr;42(2):21-8.
Time-course of induced accumulation of callose in tomato cells has been studied. Localization of callose in L. esculenthum cells was investigated by fluorescent microscopy technique, and the optimal time for its determination was found. Callose accumulation in tomato cells treated with different biotic elicitors was determined. Nonlinear dependence between callose accumulation and concentration of chitin oligomers (with 3-5 N-acetylglucosamine fragments) was established. Increasing of callose accumulation in tomato cells was proportional to the increase of concentration ofchitin dimer and chitosan in the culture medium.
研究了番茄细胞中诱导积累胼胝质的时间进程。通过荧光显微镜技术研究了番茄细胞中胼胝质的定位，并找到了其测定的最佳时间。测定了用不同生物激发子处理的番茄细胞中胼胝质的积累情况。建立了胼胝质积累与几丁质寡聚物（含3-5个N-乙酰葡糖胺片段）浓度之间的非线性关系。番茄细胞中胼胝质积累的增加与培养基中几丁质二聚体和壳聚糖浓度的增加成正比。
