Zheng Hui Ling, Yuan Chao, Bao Li Juan, An Jun Hui, Zhu Zhen Zhen, Yang Gong She
College of Animal Sci-Tech, Northwest A & F University, Yang Ling, Shaanxi Province, PR China.
Fen Zi Xi Bao Sheng Wu Xue Bao. 2008 Jun;41(3):222-6.
The recombinant adenoviruses which can overexpress fosb and Delta2Deltafosb proteins were produced. Primary calvarial osteoblasts from new born CD1 mice were infected with those adenoviruses by different proportion of Ad-Delta2Deltafosb and induced by 5 mmol/L beta-glycerophosphate, 50 microg/ml ascorbic acid and 10 nmol/L dexamethasone for 23 days and 29 days, respectively. The results by Alizarin Red Staining showed that the primary osteoblasts overexpressing Delta2Deltafosb protein formed more nodus than the control cells and the nodus in overexpressing Delta2Deltafosb protein osteoblasts were smaller than those in control cells on day 23. Differentiation of the primary osteoblasts overexpressing Delta2Deltafosb protein increased more than that of the control cells on day 29. It is an evidence for the reason that bone density of Delta2Deltafosb transgenic mice was markedly increased throughout the skeleton in new born and mature mice.
制备了能够过表达FosB和Delta2DeltaFosB蛋白的重组腺病毒。将新生CD1小鼠的原代颅骨成骨细胞用不同比例的Ad-Delta2DeltaFosB腺病毒进行感染，并分别用5 mmol/Lβ-甘油磷酸、50μg/ml抗坏血酸和10 nmol/L地塞米松诱导23天和29天。茜素红染色结果显示，在第23天，过表达Delta2DeltaFosB蛋白的原代成骨细胞形成的结节比对照细胞多，且过表达Delta2DeltaFosB蛋白的成骨细胞中的结节比对照细胞中的小。在第29天，过表达Delta2DeltaFosB蛋白的原代成骨细胞的分化比对照细胞增加得更多。这为Delta2DeltaFosB转基因小鼠在新生和成熟小鼠的整个骨骼中骨密度显著增加的原因提供了证据。
