Zou Yang, Hu Jing, Wang Zhao-Xiao, Wang Ding-Ming, Li Ming-Hui, Ren Guo-Dong, Duan Zheng-Xiu, Fu Zhen F, Plyusnin Alexander, Zhang Yong-Zhen
Beijing Friendship Hospital, Affiliate of Capital Medical University, Beijing, PR China.
Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping Liuzi 5, 102206 Beijing, PR China.
J Gen Virol. 2008 Aug;89(Pt 8):1987-1997. doi: 10.1099/vir.0.2008/000497-0.
To gain further insight into the molecular epidemiology of Hantaan virus (HTNV) in Guizhou, China, rodents were captured in this region in 2004 and 2005. In addition, serum samples were collected from four patients. Ten hantaviruses were isolated successfully in cell culture from four humans, two Apodemus agrarius, three Rattus norvegicus and one Rattus nitidus. The nucleotide sequences for their small (S), medium (M) and partial large (L) segments were determined. Phylogenetic analysis of the S and M segment sequences revealed that all of these isolates belong to the species HTNV, suggesting a spillover of HTNV from A. agrarius to Rattus rats. All available isolates from Guizhou were divided into four distinct groups either in the S segment tree or in the M segment tree. The clustering pattern of these isolates in the S segment tree was not in agreement with that in the M or L segment tree, showing that genetic reassortment between HTNV had occurred naturally. Analysis of the S segment sequences from available HTNV strains indicated that they formed three clades. The first clade, which comprised only viruses from Guizhou, was the outgroup of clades II and III. The viruses in the second clade were found in Guizhou and mainly in the far-east Asian region, including China. However, the viruses in the third clade were found in most areas of China, including Guizhou, in which haemorrhagic fever with renal syndrome (HFRS) is endemic. Our results reveal that the highest genetic diversity of HTNV is in a limited geographical region of Guizhou, and suggest that Guizhou might be a radiation centre of the present form of HTNV.
为了更深入了解中国贵州汉坦病毒（HTNV）的分子流行病学，于2004年和2005年在该地区捕获啮齿动物。此外，还收集了4名患者的血清样本。从4名人类、2只黑线姬鼠、3只褐家鼠和1只大足鼠中成功分离出10株汉坦病毒。测定了它们的小（S）、中（M）和部分大（L）片段的核苷酸序列。对S和M片段序列的系统发育分析表明，所有这些分离株均属于汉坦病毒种，提示汉坦病毒从黑线姬鼠向家鼠属发生了溢出。贵州所有可获得的分离株在S片段树或M片段树中均分为4个不同的组。这些分离株在S片段树中的聚类模式与M或L片段树中的聚类模式不一致，表明汉坦病毒之间自然发生了基因重配。对现有汉坦病毒株S片段序列的分析表明，它们形成了3个进化枝。第一个进化枝仅由来自贵州的病毒组成，是进化枝II和III的外类群。第二个进化枝中的病毒在贵州发现，主要分布在包括中国在内的远东亚洲地区。然而，第三个进化枝中的病毒在中国的大多数地区发现，包括贵州，肾综合征出血热（HFRS）在该地区为地方病。我们的结果表明，汉坦病毒的最高遗传多样性存在于贵州的一个有限地理区域，并提示贵州可能是当前汉坦病毒形式的辐射中心。
