Zaiat M, Vieira L G, Foresti E
Escola de Engenharia de São Carlos (EESC), Universidade de São Paulo (USP), Departamento de Hidráulica e Saneamento, 1465 Av. Dr. Carlos Botelho, 13560-250, São Carlos, SP, Brazil.
Biotechnol Bioeng. 1997 Jan 20;53(2):220-5. doi: 10.1002/(SICI)1097-0290(19970120)53:2<220::AID-BIT13>3.0.CO;2-O.
This article presents a method for evaluating the intrinsic kinetic parameters of the specific substrate utilization rate (r) equation and discusses the results obtained for anaerobic sludge-bed samples taken from a horizontal-flow anaerobic immobilized sludge (HAIS) reactor. This method utilizes a differential reactor filled with polyurethane foam matrices containing immobilized anaerobic sludge which is subjected to a range of feeding substrate flow rates. The range of liquid superficial velocities thus obtained are used for generating data of observed specific substrate utilization rates (r(obs)) under a diversity of external mass transfer resistance conditions. The r(obs) curves are then adjusted to permit their extrapolation for the condition of no external mass transfer resistance, and the values determined are used as a test for the condition of absence of limitation of internal mass transfer. The intrinsic parameters r(max), the maximum specific substrate utilization rate, and K(s), the half-velocity coefficient, are evaluated from the r values under no external mass transfer resistance and no internal mass transfer limitation. The application of such a method for anaerobic sludge immobilized in polyurethane foam particles treating a glucose substrate at 30 degrees C resulted in intrinsic r(max) and K(s), respectively, of 0.330 mg chemical oxygen demand (COD) . mg(-1) volatile suspended solids (VSS) . h(-1) and 72 mg COD . L(-1). In comparison with the values found in the literature, intrinsic r(max) is significantly high and intrinsic K(s) is relatively low. (c) 1997 John Wiley & Sons, Inc.
本文介绍了一种评估特定底物利用率（r）方程内在动力学参数的方法，并讨论了从水平流厌氧固定化污泥（HAIS）反应器中采集的厌氧污泥床样品所获得的结果。该方法利用一个装有含有固定化厌氧污泥的聚氨酯泡沫基质的微分反应器，使其承受一系列进料底物流速。由此获得的液体表观流速范围用于生成在多种外部传质阻力条件下观察到的特定底物利用率（r(obs)）数据。然后对r(obs)曲线进行调整，以便将其外推到无外部传质阻力的条件，所确定的值用作无内部传质限制条件的检验。内在参数r(max)（最大特定底物利用率）和K(s)（半速度系数）是根据无外部传质阻力和无内部传质限制时的r值评估的。这种方法应用于在30℃下处理葡萄糖底物的聚氨酯泡沫颗粒固定化厌氧污泥时，分别得到内在r(max)和K(s)为0.330毫克化学需氧量（COD）·毫克⁻¹挥发性悬浮固体（VSS）·小时⁻¹和72毫克COD·升⁻¹。与文献中发现的值相比，内在r(max)显著较高，而内在K(s)相对较低。（c）1997约翰威立父子公司。
