Arany Zoltan P
Cardiovascular Institute, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, Massachusetts, USA.
Curr Protoc Hum Genet. 2008 Jul;Chapter 11:Unit 11.10. doi: 10.1002/0471142905.hg1110s58.
Recent technical advances in quantitative real-time PCR (qRT-PCR) have allowed for extensive miniaturization, thereby rendering the technique amenable to high-throughput assays. Large numbers of different nucleic acids can now rapidly be measured quantitatively. Many investigations can benefit from this approach, including determination of gene expression in hundreds of samples, determination of hundreds of genes in a few samples, or even quantification of nucleic acids other than mRNA. A simple technique is described here to quantify 1880 transcripts of choice from any number of starting RNA samples.
定量实时聚合酶链反应（qRT-PCR）技术的最新进展实现了大规模微型化，从而使该技术适用于高通量检测。现在可以快速对大量不同的核酸进行定量测量。许多研究都能从这种方法中受益，包括测定数百个样本中的基因表达、测定少数样本中的数百个基因，甚至对mRNA以外的核酸进行定量。本文介绍了一种简单的技术，可从任意数量的起始RNA样本中对1880个选定的转录本进行定量。
