Grankvist O, Gustafsson A, Bredberg-Rådén U, Pallangyo K, Mhalu F, Biberfeld P, Biberfeld G, Wadell G
Department of Virology, University of Umeå, Sweden.
AIDS. 1991 May;5(5):575-8.
In order to facilitate the detection of integrated HIV-1 proviral DNA from African as well as European patients, four new primer pairs for use in the polymerase chain reaction (PCR), localised in the gag, pol, vif and env genes of HIV-1, were constructed. The primer pairs were compared to all accessible HIV-1 sequences from African and European isolates and to some of the earlier published and most commonly used primer pairs. HIV-1 DNA was detected in blood drawn from 13 out of 13 individuals infected in Africa, in three out of three Tanzanian HIV-1 isolates and in three out of three asymptomatic Swedes infected in Europe. The new selection of primer pairs can be used as an alternative to enhance the detection of HIV-1 of different origins.
为便于检测来自非洲和欧洲患者的整合型HIV-1前病毒DNA，构建了4对用于聚合酶链反应（PCR）的新引物对，它们位于HIV-1的gag、pol、vif和env基因中。将这些引物对与来自非洲和欧洲分离株的所有可获取的HIV-1序列以及一些早期发表且最常用的引物对进行了比较。在从非洲感染的13名个体中抽取的血液中检测到了HIV-1 DNA，在3株坦桑尼亚HIV-1分离株中检测到了HIV-1 DNA，在欧洲感染的3名无症状瑞典人中也检测到了HIV-1 DNA。新选择的引物对可作为一种替代方法，用于增强对不同来源HIV-1的检测。
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