Cohen B, Wimmer E A, Cohen S M
Howard Hughes Medical Institute, Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Mech Dev. 1991 Mar;33(3):229-40. doi: 10.1016/0925-4773(91)90030-a.
The development of the leg and wing primordia in the Drosophila embryo has been traced using molecular markers. Distal-less and disconnected gene expression provide molecular labels for the leg primordia throughout embryonic development, disconnected expression in the developing leg primordia depends on Distal-less activity. The leg primordia arise as discrete clusters of cells that occupy well defined positions in the embryonic ectoderm. At later stages of embryogenesis the primordia become morphologically recognizable and are intimately associated with the development of the Keilin's organs. The presumptive leg disc and the Keilin's organ appear to derive from a common primordium. Similarly the Abnormal leg pattern gene provides a molecular label for the wing and haltere primordia. The dorsal thoracic primordia appear to be of independent origin from the legs.
利用分子标记追踪了果蝇胚胎中腿和翅原基的发育过程。Distal-less和disconnected基因的表达为整个胚胎发育过程中的腿原基提供了分子标记，发育中的腿原基中的disconnected表达依赖于Distal-less的活性。腿原基以离散的细胞簇形式出现，这些细胞簇在胚胎外胚层中占据明确的位置。在胚胎发育的后期阶段，原基在形态上变得可识别，并与凯林器官的发育密切相关。假定的腿盘和凯林器官似乎起源于共同的原基。同样，异常腿型基因也为翅和平衡棒原基提供了分子标记。胸部背侧原基似乎与腿起源独立。
